HK-CRISPR-Nup62-mEGFP Cells

The HK-CRISPR-Nup62-mEGFP cell line is a human cell model with the Nup62 gene tagged with monomeric Enhanced Green Fluorescent Protein (mEGFP). This modification allows for real-time visualization of Nup62 within the nuclear envelope, aiding studies on nuclear transport, NPC assembly, and dynamics. Precise genome editing was achieved using CRISPR-Cas9 technology, making it a reliable model for investigating Nup62's role in cellular processes.

This cell line is valuable for research in nucleocytoplasmic transport, cell cycle regulation, and nuclear architecture. The fluorescent tagging of Nup62 enables high-resolution imaging and live-cell tracking, facilitating fluorescence microscopy and other imaging techniques. Researchers can explore the molecular mechanisms of nuclear-cytoplasmic exchange and the role of Nup62 in diseases such as cancer and neurodegenerative disorders.