HK-CRISPR-Nup93-mEGFP Cells

The HK-CRISPR-Nup93-mEGFP cell line is derived from Hela Kyoto cells and engineered using CRISPR/Cas9 technology to express Nup93 fused with monomeric enhanced green fluorescent protein (mEGFP). This allows for real-time visualization of Nup93 within the nuclear envelope, aiding in the study of nuclear-cytoplasmic transport, nuclear pore complex assembly, and nuclear envelope integrity.
Nup93 is crucial for maintaining nuclear pore complex architecture and function. The mEGFP tag enables tracking its dynamics and interactions, facilitating high-resolution imaging techniques like confocal microscopy. This cell line helps researchers understand gene regulation, nucleocytoplasmic trafficking, and cellular stress responses.
The HK-CRISPR-Nup93-mEGFP cell line is a valuable resource for studying cell biology and nuclear pore complex-associated diseases, contributing to potential therapeutic strategies targeting nuclear transport pathways. It is particularly useful for exploring the nuclear envelope's role in cellular function and pathology.