HK-CRISPR-Pom121-mCherry Cells

The HK-CRISPR-Pom121-mCherry cell line is a genetically engineered variant of HeLa Kyoto cells, designed for advanced cellular research. These cells have been modified using CRISPR-Cas9 technology to add a fluorescent mCherry tag to the Pom121 gene, a key component of the nuclear pore complex (NPC). This modification allows researchers to visualize Pom121 in real-time, aiding studies of nuclear dynamics and NPC functions.

The mCherry tag, emitting red fluorescence, facilitates detailed examination of NPC behavior and nuclear-cytoplasmic transport using fluorescence microscopy. This cell line is a valuable tool for investigating nuclear pore proteins, gene expression regulation, cell cycle control, and signal transduction.