HK-CRISPR-CAP-H2-mEGFP Cells

The HK-CRISPR-CAP-H2-mEGFP cell line is a Hela Kyoto cell model developed using CRISPR-Cas9 technology. This cell line incorporates the mEGFP (monomeric Enhanced Green Fluorescent Protein) into the CAP-H2 gene, which is part of the condensin II complex involved in chromosome segregation and condensation during mitosis. The mEGFP tag allows researchers to visually track condensin II dynamics during cell division.

This cell line is useful for studying mitotic processes, chromosome architecture, and gene regulation. The mEGFP marker enables live-cell imaging and real-time observation of CAP-H2 protein function. This model helps investigate the molecular mechanisms of cell cycle progression and chromosomal integrity, aiding in the understanding of genetic disorders and the development of therapeutic strategies.