HK-CRISPR-mEGFP-RanBP2/Nup358 Cells

"The HK-CRISPR-mEGFP-RanBP2/Nup358 cell line is a genetically engineered derivative of the HeLa Kyoto cell line. This model has been specifically modified using CRISPR/Cas9 technology to express the mEGFP (monomeric Enhanced Green Fluorescent Protein) tag fused to the RanBP2/Nup358 protein. RanBP2/Nup358 is a major component of the nuclear pore complex, playing a crucial role in nucleocytoplasmic transport, mitotic progression, and regulation of various signaling pathways.

By tagging RanBP2/Nup358 with mEGFP, researchers can visually track the localization and dynamics of this protein in live cells, providing valuable insights into its function and interaction with other cellular components. The green fluorescence of mEGFP allows for real-time imaging and analysis using fluorescence microscopy, facilitating studies on nuclear transport mechanisms, cell cycle regulation, and the impact of RanBP2/Nup358 on cellular homeostasis. This cell line serves as a powerful tool for investigating the molecular mechanisms underpinning nuclear-cytoplasmic trafficking and its implications in cellular physiology and pathology."