HK Mad2-LAP/H2B-mCherry Cells

The HK Mad2-LAP/H2B-mCherry cell line is a genetically engineered cell model extensively utilized for studying chromosome segregation and the spindle assembly checkpoint during mitosis. These cells are derived from HeLa Kyoto cells, a robust human cell line originally taken from a cervical carcinoma. The HK Mad2-LAP (LAP-tagged Mad2) aspect of the cell line facilitates the visualization and functional analysis of the Mad2 protein, a critical component of the spindle assembly checkpoint that prevents anaphase onset until all chromosomes are properly aligned at the metaphase plate.
Incorporation of H2B-mCherry, where histone H2B is tagged with the mCherry fluorescent protein, allows for real-time imaging of chromatin dynamics during cell division. This feature makes the HK Mad2-LAP/H2B-mCherry cell line an excellent tool for high-resolution live-cell imaging techniques to observe chromosomal movements and mitotic progression in human cells under various experimental conditions. The use of fluorescent tags aids in precise tracking and quantification, thereby providing valuable insights into the molecular mechanisms governing cell cycle regulation and chromosomal stability.